Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/eLife.52709

Figure Lengend Snippet: Time-course analysis of serum levels of intact FGF23 for three consecutive phytate doses. Statistical significance was tested by repeated measure ANOVA between subjects and groups. All data are presented as the mean ± SD for each dose (n = 6 per dose).

Article Snippet: Rat intact PTH (Immutopics, San Clemente, CA, USA), human intact PTH (Abcam), soluble RANKL (Immundiagnostik, Bensheim, Germany), osteoprotegerin (Alpco Immunoassay, Salem, NH, USA), rat intact and C-terminal FGF23 (Elabscience, Houston, TX, USA), and human intact FGF23 (Elabscience) were measured using enzyme-linked immunosorbent assay.

Techniques:

Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/eLife.52709

Figure Lengend Snippet: ( A–C ) Time-course analysis of serum levels of intact PTH ( A ), 25(OH)D ( B ), and 1,25(OH) 2 D ( C ) in rats fed control, HP-LCa 2+ , and HP-HCa 2+ diets. For the early measurement of 25(OH)D ( B ) and 1,25(OH) 2 D, we pooled the sera from 2 to 3 rats. ( D–F ) Time-course analysis of renal CYP27B1 ( D ), CYP24A1 ( E ), and VDR ( F ) in rats fed control, HP-LCa 2+ , and HP-HCa 2+ diets. ( G ) Immunoblot analysis of renal αKlotho, NHERF1, NaPi-2a in rats fed control, HP-LCa 2+ , and HP-HCa 2+ diets. ( H ) The levels of renal proteins were quantified using ImageJ software (NIH, Bethesda, MD, USA). ( I ) Immunoblot analysis of calvarial FGF23 in rats fed control, HP-LCa 2+ , and HP-HCa 2+ diets. ( J ) The levels of total calvarial FGF23 were quantified using ImageJ software. All comparisons were conducted using two-way ANOVA with Tukey’s post hoc multiple comparison testing or the Kruskal–Wallis test ( A–C ) for non-parametric statistical analysis of data that did not have a normal distribution. Panels ( A–C ) failed the normality test, but no outliers were identified. All data are presented as the mean ± SD of each group (n = 4–6 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls.

Article Snippet: Rat intact PTH (Immutopics, San Clemente, CA, USA), human intact PTH (Abcam), soluble RANKL (Immundiagnostik, Bensheim, Germany), osteoprotegerin (Alpco Immunoassay, Salem, NH, USA), rat intact and C-terminal FGF23 (Elabscience, Houston, TX, USA), and human intact FGF23 (Elabscience) were measured using enzyme-linked immunosorbent assay.

Techniques: Control, Western Blot, Software, Comparison

Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/eLife.52709

Figure Lengend Snippet: ( A ) The renal expression of downregulated genes associated with Ca 2+ and phosphate homeostasis in rats fed control or phytate-supplemented diets (n = 8 per group). ( B ) Results of immunoblot analyses and protein levels of renal aKlotho, NHERF1, and NaPi-2a after 2 weeks for rats fed control, HP- LCa 2+ , and HP-HCa 2+ diets. ( C ) The levels of renal proteins were quantified using ImageJ software (n = 6 per group). ( D, E ) Time course analysis of serum levels of intact FGF23 ( D ) and C-terminal FGF23 ( E ) in rats fed control, HP-LCa 2+ , and HP-HCa 2+ diets. ( F ) Representative immunohistochemical staining of EDTA-decalcified femur sections for FGF23 from rats fed control, HP-LCa 2+ , and HP-HCa 2+ diets. All data are presented as the mean ± SD of each group (n = 3–8 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls.

Article Snippet: Rat intact PTH (Immutopics, San Clemente, CA, USA), human intact PTH (Abcam), soluble RANKL (Immundiagnostik, Bensheim, Germany), osteoprotegerin (Alpco Immunoassay, Salem, NH, USA), rat intact and C-terminal FGF23 (Elabscience, Houston, TX, USA), and human intact FGF23 (Elabscience) were measured using enzyme-linked immunosorbent assay.

Techniques: Expressing, Control, Western Blot, Software, Immunohistochemical staining, Staining

Journal: eLife

Article Title: High-phytate/low-calcium diet is a risk factor for crystal nephropathies, renal phosphate wasting, and bone loss

doi: 10.7554/eLife.52709

Figure Lengend Snippet:

Article Snippet: Rat intact PTH (Immutopics, San Clemente, CA, USA), human intact PTH (Abcam), soluble RANKL (Immundiagnostik, Bensheim, Germany), osteoprotegerin (Alpco Immunoassay, Salem, NH, USA), rat intact and C-terminal FGF23 (Elabscience, Houston, TX, USA), and human intact FGF23 (Elabscience) were measured using enzyme-linked immunosorbent assay.

Techniques: Control, Enzyme-linked Immunosorbent Assay, RIA Assay, Staining, Colorimetric Assay, Blocking Assay, Recombinant, In Vitro, Software